VWR International 1325F Manuel d'utilisateur Page 128
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Electroporation
Reagents
126
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Description Pk Cat. No.
BTXpress™ solution: 5 ml bottle for up to 50 reactions (without cuvettes) 5 ml
732-1284
BTXpress™ solution: 10 ml bottle for up to 100 reactions (without cuvettes) 10 ml
732-1285
Kit: 5 ml BTXpress™ solution and 50× 2 mm gap cuvettes with transfer pipettes, 50 reactions 1 KIT
732-1280
Kit: 5 ml BTXpress™ solution and 20× 4 mm gap cuvettes with transfer pipettes, 20 reactions 1 KIT
732-1281
Kit: 10 ml BTXpress™ solution and 100× 2 mm gap cuvettes with transfer pipettes, 100 reactions 1 KIT
732-1282
Kit: 10 ml BTXpress™ solution and 40× 4 mm gap cuvettes with transfer pipettes, 40 reactions 1 KIT 732-1283
Electroporation solution kits, BTXpress™
BTX
The BTXpress™ is a single buffer solution, developed to quickly and efficiently deliver genes
into mammalian cells that were previously considered “hard to transfect” by chemical and
other non viral methods. This solution, in combination with the BTX electroporation
instruments, provides researchers with the versatility and success needed across a broad
range of cell types, while maintaining critical cell viability. Transfection using this high
performance electroproation solution is equally effective in delivering DNA as well as siRNA
into mammalian cells.
As a universal solution the BTXpress™ electroporation reagent can be used with
electroporation generators, including Amaxa™ Nucleofector®, to deliver similar to equal
performance without the cost associated with other systems.
• High efficiency transfection of cell lines and previously considered “hard to transfect” cells
• Low toxicity resulting in improved cell viability
• One buffer used in place of standard electroporation buffers for all mammalian cell types
• Versatility of choosing electroporation optimisation settings for the highest transfection efficiency with BTX instruments
• Increased numbers of tranfections per kit providing better value
Delivery information: BTXpress™ high performance electroporation solution is supplied either as a kit, which includes the BTX Plus cuvettes
with transfer pipettes, or as a buffer alone.
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- Nucleic Acid Purification 10
- THE CHEMICAL BRAND 11
- FROM VWR INTERNATIONAL 11
- E.Z.N.A.® Blood DNA Kits 12
- 11All You Need Genomics 13
- 13All You Need Genomics 15
- E.Z.N.A.® Tissue DNA kits 15
- 21All You Need Genomics 23
- E.Z.N.A.® miRNA Isolation kit 23
- E.Z.N.A.® MagBind® miRNA kits 23
- 23All You Need Genomics 25
- E.Z.N.A.® Total RNA kits 25
- Total RNA Reagents 29
- VWR by Omega bio-tek 29
- Phase Lock Gel™ 30
- DNA/RNA Clean Up 35
- GE Healthcare 35
- 35All You Need Genomics 37
- FTA® Cards 37
- FTA® Elute Cards 38
- FTA® reagent and accessories 38
- Modifying Enzymes 41
- Micrococcus 42
- Tritirachium album 43
- DNA polymerase 46
- 45All You Need Genomics 47
- DNA polymerase MasterMixes 48
- 47All You Need Genomics 49
- PerfectTaq™ DNA polymerase 49
- MasterMix 49
- MasterTaq kits 50
- Hot Start PCR Reagents 51
- Maxima™ Hot Start 52
- DNA polymerase, Fermentas 52
- 51All You Need Genomics 53
- HotMasterMix 53
- 53All You Need Genomics 55
- Exact polymerase 55
- AccuPOL DNA polymerase 55
- PCR Extender system 58
- All You Need Genomics 63
- PCR Tubes 63
- PCR Strips 64
- Caps for the PCR strips tubes 65
- PCR seals 70
- Heat Sealers 72
- Thermo Scientific 72
- PCR Workstations 74
- 73All You Need Genomics 75
- PCR Cyclers 76
- Real Time PCR 79
- Masterscript kit 80
- 79All You Need Genomics 81
- 81All You Need Genomics 83
- 85All You Need Genomics 87
- PerfeCTa® qPCR FastMix® II 88
- PerfeCTa® qPCR ToughMix™ 88
- 87All You Need Genomics 89
- 89All You Need Genomics 91
- Reagents & Buffers 100
- VWR COLLECTION 102
- 101All You Need Genomics 103
- Electrophoresis 103
- DNA ladder, 100 bp 103
- DNA ladder, 1 kb 103
- DNA MicroMarker™ 104
- 103All You Need Genomics 105
- RNA ladder, 1 kb, RiboReady™ 105
- Ethidium bromide 105
- TALKING YOUR LANGUAGE 106
- 105All You Need Genomics 107
- 107All You Need Genomics 109
- 109All You Need Genomics 111
- 111All You Need Genomics 113
- 113All You Need Genomics 115
- Power supplies, Power Source 115
- PCR Cloning 116 117
- >lXiXek\\[ 119
- RTGEKUG 120
- RGTHQTOCPEG 120
- Transfection 121
- 121All You Need Genomics 123
- INSTRUMENT SERVICE 125
- AND MAINTENANCE 125
- (14.+37+&*#0&.+0) 126
- Electroporation 127
- E-BUSINESS 128
- 127All You Need Genomics 129
- Electroporator, ECM® 630 129
- Electroporation cuvettes 129
- 129All You Need Genomics 131
- 131All You Need Genomics 133
- Electrodes, Genetrodes™ 133
- Electrodes, Genepaddles 133
- Electrodes, Tweezertrodes™ 133
- Caliper electrodes 134
- Electrodes, 2-Needle Array™ 134
- 133All You Need Genomics 135
- Detection 137
- 139All You Need Genomics 141
- /QDKNHWPMOCZa/KP 144
- '7466%OKP 144
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