VWR International 1325F Manuel d'utilisateur Page 54

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PCR
Direct PCR Reagents
52
www.vwr.com
Description Pk Cat. No.
PCR directly from plants
Phire® Plant Direct PCR kit, 200 reactions (50 µl each) 1KIT
ADVAF-130
PCR directly from animal tissues
Phire® Animal Tissue Direct PCR kit, 200 reactions (50 µl each) 1KIT
ADVAF-140
PCR directly from human sample types
Phusion® Human Specimen Direct PCR kit, 200 reactions (20 µl each) 1 KIT
ADVAF-150
PCR directly from whole blood
Phusion® Blood Direct PCR kit, F-547L, 500 reactions (20 µl each) 1 KIT
ADVAF-547L
Phusion® Blood Direct PCR kit, F-547S, 100 reactions (20 µl each) 1 KIT
ADVAF-547S
E-BUSINESS
More than just a webshop for all of your laboratory needs Please visit us at: http://eu.vwr.com/e-business
Direct PCR kits
Thermo Scientific
Direct PCR kits save time and cost by allowing amplification of DNA directly from the source material. Direct PCR is based on Thermo
Scientific Phire® Hot Start II DNA polymerase and Phusion® Hot Start II High-Fidelity DNA polymerase. These fusion enzymes are
exceptionally fast, robust and highly tolerant of many PCR inhibitors present in unpurified sample material. This allows reliable amplification in
challenging conditions where conventional DNA polymerases are completely inhibited.
No need for time consuming and expensive DNA purification steps
Very little sample material required
Simple protocols with minimal hands-on time
Short PCR protocol times
Tested with wide variety of sample types
Robust Hot Start DNA polymerases guarantee high yields of specific product
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