VWR International 1325F Manuel d'utilisateur Page 81
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79All You Need Genomics
Real Time PCR
cDNA Synthesis
Description Pk Cat. No.
M-MuLV Reverse Transcriptase, 5000 units (20 U/µl) 1KIT
FERMEP0351
Activity, %
Time, min
20
0
40
60
80
100
0 5 30 60 120 240
Maxima
™
RT
M-MuLV RT H Minus
M-MuLV RT
Δ
Rn
Cycle number
-0.02
0.00
0.04
0.08
0.12
0.16
0.20
0.24
0.28
A
2 6 10 14 18 22 26 30 34 38
Cycle
Quantity, pg
10 10
1
10
2
10
3
10
4
10
5
10
6
10
7
17
19
21
23
25
27
31
35
37
29
33
39
B
M-MuLV Reverse Transcriptase, Fermentas
Description Pk Cat. No.
Maxima™ Reverse Transcriptase, 2000 units (200 U/µl) 1KIT
FERMEP0741
Maxima™ Reverse Transcriptase, 10 000 units (200 U/µl) 1KIT FERMEP0742
Maxima™ Reverse Transcriptase, 4×10 000 units (200 U/µl) 1KIT
FERMEP0743
Thermo Scientific
Maxima™ Reverse Transcriptase, Fermentas
Description Pk Cat. No.
Maxima® First Strand cDNA Synthesis kit for RT-qPCR, 50 reactions 1 KIT
FERMK1641
Maxima® First Strand cDNA Synthesis kit for RT-qPCR, 200 reactions 1 KIT
FERMK1642
M-MuLV Reverse Transcriptase (RT) is an RNA- and DNA-dependent DNA polymerase. It can use either RNA or DNA to prime DNA
synthesis. Applications include first strand cDNA synthesis for RT-PCR and real time RT-PCR, synthesis of cDNA for cloning and expression,
and generation of labelled cDNA probes for microarrays.
Thermo Scientific
Maxima™ First Strand cDNA Synthesis kit, Fermentas
• High yields of first strand cDNA up to 9 kb
• Optimum activity at 37 °C
• Incorporates modified nucleotides (e.g., Cy3-, Cy5-, rhodamine-, aminoallyl-, fluorescein-labelled nucleotides)
Maxima™ Reverse Transcriptase is an advanced enzyme derived by
in vitro
evolution of M-
MuLV RT for RT-qPCR application. The enzyme features high thermostability, robustness and
increased synthesis rates in RT-qPCR. The Maxima™ Reverse Transcriptase is capable of
cDNA synthesis at elevated temperatures (50 to 60 °C) and completion of the reverse
transcription reaction in 15 to 30 minutes. The use of this enzyme in combination with
Maxima™ qPCR Master Mixes ensures high specificity, sensitivity and wide linear range of
two step RT-qPCR. Applications include first strand cDNA synthesis, RT-PCR, and RT-qPCR.
Thermo Scientific
• Thermostable – 90% active after incubation at 50 °C for 60 minutes in a reaction mixture
•Activeupto60°C
• High yields of full-length cDNA as long as 20 kb
• Efficient – complete cDNA synthesis in 15 to 30 minutes
• Incorporates modified nucleotides
• Developed through
in vitro
evolution
The Maxima™ First Strand cDNA Synthesis kit is a convenient system optimised for use as a first step in two step RT-qPCR. The kit includes
the Maxima™ Reverse Transcriptase, which is an advanced enzyme derived by
in vitro
evolution of M-MuLV RT for RT-qPCR application. The
enzyme features high thermostability, robustness and increased synthesis rates in RT-qPCR. The Maxima™ First Strand cDNA Synthesis kit
is capable of cDNA synthesis at elevated temperatures (50 to 60 °C) and completion of the reverse transcription reaction in 15 minutes. In
addition, the kit offers highly convenient composition, where Maxima™ Reverse Transcriptase and RiboLock™ RNase Inhibitor are premixed,
while all other reaction components: reaction buffer, dNTPs and primers (random hexamers and Oligo(dT)₁₈) are premixed in another tube as
a 5X reaction mix.
Delivery information: Supplied with 5X RT Buffer.
• Increased reaction temperatures – up to 60 °C
• Premixed components for convenient use in RT-qPCR
• Increased synthesis rate – complete cDNA synthesis in 15 minutes
• High sensitivity and specificity
Delivery information: Kit includes Maxima™ enzyme mix, 5X reaction mix, nuclease-free water, and detailed protocol.
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- Nucleic Acid Purification 10
- THE CHEMICAL BRAND 11
- FROM VWR INTERNATIONAL 11
- E.Z.N.A.® Blood DNA Kits 12
- 11All You Need Genomics 13
- 13All You Need Genomics 15
- E.Z.N.A.® Tissue DNA kits 15
- 21All You Need Genomics 23
- E.Z.N.A.® miRNA Isolation kit 23
- E.Z.N.A.® MagBind® miRNA kits 23
- 23All You Need Genomics 25
- E.Z.N.A.® Total RNA kits 25
- Total RNA Reagents 29
- VWR by Omega bio-tek 29
- Phase Lock Gel™ 30
- DNA/RNA Clean Up 35
- GE Healthcare 35
- 35All You Need Genomics 37
- FTA® Cards 37
- FTA® Elute Cards 38
- FTA® reagent and accessories 38
- Modifying Enzymes 41
- Micrococcus 42
- Tritirachium album 43
- DNA polymerase 46
- 45All You Need Genomics 47
- DNA polymerase MasterMixes 48
- 47All You Need Genomics 49
- PerfectTaq™ DNA polymerase 49
- MasterMix 49
- MasterTaq kits 50
- Hot Start PCR Reagents 51
- Maxima™ Hot Start 52
- DNA polymerase, Fermentas 52
- 51All You Need Genomics 53
- HotMasterMix 53
- 53All You Need Genomics 55
- Exact polymerase 55
- AccuPOL DNA polymerase 55
- PCR Extender system 58
- All You Need Genomics 63
- PCR Tubes 63
- PCR Strips 64
- Caps for the PCR strips tubes 65
- PCR seals 70
- Heat Sealers 72
- Thermo Scientific 72
- PCR Workstations 74
- 73All You Need Genomics 75
- PCR Cyclers 76
- Real Time PCR 79
- Masterscript kit 80
- 79All You Need Genomics 81
- 81All You Need Genomics 83
- 85All You Need Genomics 87
- PerfeCTa® qPCR FastMix® II 88
- PerfeCTa® qPCR ToughMix™ 88
- 87All You Need Genomics 89
- 89All You Need Genomics 91
- Reagents & Buffers 100
- VWR COLLECTION 102
- 101All You Need Genomics 103
- Electrophoresis 103
- DNA ladder, 100 bp 103
- DNA ladder, 1 kb 103
- DNA MicroMarker™ 104
- 103All You Need Genomics 105
- RNA ladder, 1 kb, RiboReady™ 105
- Ethidium bromide 105
- TALKING YOUR LANGUAGE 106
- 105All You Need Genomics 107
- 107All You Need Genomics 109
- 109All You Need Genomics 111
- 111All You Need Genomics 113
- 113All You Need Genomics 115
- Power supplies, Power Source 115
- PCR Cloning 116 117
- >lXiXek\\[ 119
- RTGEKUG 120
- RGTHQTOCPEG 120
- Transfection 121
- 121All You Need Genomics 123
- INSTRUMENT SERVICE 125
- AND MAINTENANCE 125
- (14.+37+&*#0&.+0) 126
- Electroporation 127
- E-BUSINESS 128
- 127All You Need Genomics 129
- Electroporator, ECM® 630 129
- Electroporation cuvettes 129
- 129All You Need Genomics 131
- 131All You Need Genomics 133
- Electrodes, Genetrodes™ 133
- Electrodes, Genepaddles 133
- Electrodes, Tweezertrodes™ 133
- Caliper electrodes 134
- Electrodes, 2-Needle Array™ 134
- 133All You Need Genomics 135
- Detection 137
- 139All You Need Genomics 141
- /QDKNHWPMOCZa/KP 144
- '7466%OKP 144
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