VWR International 1325F Manuel d'utilisateur Page 30
- Page / 144
- Table des matières
- MARQUE LIVRES
Noté. / 5. Basé sur avis des utilisateurs
Nucleic Acid Purification
Total RNA Reagents
28
www.vwr.com
PLG Heavy
Aqueous phase High density sample (for example, high salt content)
Organic phase
Standard mixtures of phenol, chloroform, isoamyl alcohol with a chloroform fraction of at least 60% (Phenol as the
sole organic solvent is not compatible with PLG Heavy)
PLG Light
Aqueous phase Low-density sample
Organic phase Standard mixtures of phenol, chloroform, isoamyl alcohol
Sample material PLG type recommended
Genomic DNA from mouse tails Use PLG Heavy to enhance isolation using a standard proteinase K/SDS organic extraction protocol
Plasmid DNA isolation Use PLG Heavy to enhance isolation using standard alkaline lysis/phenol extraction procedures
Total RNA isolation
Use PLG Heavy to enhance isolation from cells and tissues using a modified guanidinium isothiocyanate/acid phenol
method
Genomic DNA isolation Use PLG Light to enhance isolation using standard proteinase K/SDS procedures
DNA isolation from agarose gels Use PLG Light to enhance isolation of DNA fragments using phenol extraction procedures
Lambda Phage or M13 DNA isolation Use PLG Light to enhance isolation of bacteriophage DNA using standard purification protocols
Description Pk Cat. No.
Phase Lock Gel Light
Tube size 1,5 ml for 100 - 500 µl sample size, 200 tubes 200
713-2533
Tube size 2,0 ml for 100 - 750 µl sample size, 200 tubes 200
713-2535
Tubesize15mlfor1-6mlsamplesize,100tubes 100
713-2537
Tube size 50 ml for 5 - 20 ml sample size, 25 tubes 25
713-2539
Phase Lock Gel Heavy
Tube size 2,0 ml for 100 - 750 µl sample size, 200 tubes 200
713-2536
Tubesize15mlfor1-6mlsamplesize,100tubes 100
713-2538
Tube size 50 ml for 5 - 20 ml sample size, 25 tubes 25
713-2540
Tube size 1,5 ml for 100 - 500 µl sample size, 200 tubes 200
713-2534
Phase Lock Gel™
5PRIME
Phase Lock Gel (PLG) eliminates interphase-protein contamination during phenol extraction
and ensures faster results with improved recoveries. PLG migrates to form a tight seal
between the phases of an aqueous/organic extraction during centrifugation. The organic
phase and the interphase materials are effectively trapped in or below the barrier, thus
enabling complete and easy decanting or pipetting of the entire aqueous phase. The benefits
are increased yields of up to 30%, increased protection from exposure to hazardous
compounds, and no risk of interphase sample contamination. For convenience, PLG is
provided pre dispensed into standard centrifuge tubes of various sizes.
PLG is available in two different density formulations – Heavy and Light – and it can be used
in virtually any protocol that calls for extraction with phenol and/or chloroform, simply by
selecting the formulation suitable for the organic and the aqueous phases.
• Yield of nucleic acids increased by up to 30% compared to conventional organic extraction
• Eliminates interphase contamination of nucleic acid solution
• Stable gel barrier allows easy sample decanting
• Reduced contact with hazardous organic solvents
- #..;170''& 1
- +0641&7%6+10 2
- 6#$.'1(%106'065 2
- )'01/+%51.76+105 3
- KLJKWKURXJKSXWVHTXHQFLQJ 4
- 0WENGKE#EKF2WTKƂECVKQP 7
- Nucleic Acid Purification 10
- THE CHEMICAL BRAND 11
- FROM VWR INTERNATIONAL 11
- E.Z.N.A.® Blood DNA Kits 12
- 11All You Need Genomics 13
- 13All You Need Genomics 15
- E.Z.N.A.® Tissue DNA kits 15
- 21All You Need Genomics 23
- E.Z.N.A.® miRNA Isolation kit 23
- E.Z.N.A.® MagBind® miRNA kits 23
- 23All You Need Genomics 25
- E.Z.N.A.® Total RNA kits 25
- Total RNA Reagents 29
- VWR by Omega bio-tek 29
- Phase Lock Gel™ 30
- DNA/RNA Clean Up 35
- GE Healthcare 35
- 35All You Need Genomics 37
- FTA® Cards 37
- FTA® Elute Cards 38
- FTA® reagent and accessories 38
- Modifying Enzymes 41
- Micrococcus 42
- Tritirachium album 43
- DNA polymerase 46
- 45All You Need Genomics 47
- DNA polymerase MasterMixes 48
- 47All You Need Genomics 49
- PerfectTaq™ DNA polymerase 49
- MasterMix 49
- MasterTaq kits 50
- Hot Start PCR Reagents 51
- Maxima™ Hot Start 52
- DNA polymerase, Fermentas 52
- 51All You Need Genomics 53
- HotMasterMix 53
- 53All You Need Genomics 55
- Exact polymerase 55
- AccuPOL DNA polymerase 55
- PCR Extender system 58
- All You Need Genomics 63
- PCR Tubes 63
- PCR Strips 64
- Caps for the PCR strips tubes 65
- PCR seals 70
- Heat Sealers 72
- Thermo Scientific 72
- PCR Workstations 74
- 73All You Need Genomics 75
- PCR Cyclers 76
- Real Time PCR 79
- Masterscript kit 80
- 79All You Need Genomics 81
- 81All You Need Genomics 83
- 85All You Need Genomics 87
- PerfeCTa® qPCR FastMix® II 88
- PerfeCTa® qPCR ToughMix™ 88
- 87All You Need Genomics 89
- 89All You Need Genomics 91
- Reagents & Buffers 100
- VWR COLLECTION 102
- 101All You Need Genomics 103
- Electrophoresis 103
- DNA ladder, 100 bp 103
- DNA ladder, 1 kb 103
- DNA MicroMarker™ 104
- 103All You Need Genomics 105
- RNA ladder, 1 kb, RiboReady™ 105
- Ethidium bromide 105
- TALKING YOUR LANGUAGE 106
- 105All You Need Genomics 107
- 107All You Need Genomics 109
- 109All You Need Genomics 111
- 111All You Need Genomics 113
- 113All You Need Genomics 115
- Power supplies, Power Source 115
- PCR Cloning 116 117
- >lXiXek\\[ 119
- RTGEKUG 120
- RGTHQTOCPEG 120
- Transfection 121
- 121All You Need Genomics 123
- INSTRUMENT SERVICE 125
- AND MAINTENANCE 125
- (14.+37+&*#0&.+0) 126
- Electroporation 127
- E-BUSINESS 128
- 127All You Need Genomics 129
- Electroporator, ECM® 630 129
- Electroporation cuvettes 129
- 129All You Need Genomics 131
- 131All You Need Genomics 133
- Electrodes, Genetrodes™ 133
- Electrodes, Genepaddles 133
- Electrodes, Tweezertrodes™ 133
- Caliper electrodes 134
- Electrodes, 2-Needle Array™ 134
- 133All You Need Genomics 135
- Detection 137
- 139All You Need Genomics 141
- /QDKNHWPMOCZa/KP 144
- '7466%OKP 144
© 2020, manymanuals.fr. Tous droits réservés | 0.149 s |
Manymanuals.com
Manymanuals.de
Manymanuals.fr
Manymanuals.it
Manymanuals.pl
Manymanuals.cz
Manymanuals.es
Manymanuals-pt.com
Commentaires sur ces manuels