VWR International 1325F Manuel d'utilisateur

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Page 1 - #..;170''&

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Page 2 - 6#$.'1(%106'065

Nucleic Acid PurificationEndotoxin-Free Plasmid Isolation8www.vwr.comDescription Pk Cat. No.PerfectPrep™ EndoFree Plasmid Maxi kit, 10 preps 10 Tests7

Page 3 - )'01/+%51.76+105

ElectrophoresisReagents & Buffers98www.vwr.comDescription Pk Cat. No.Agarose I™ 100 g0710-100GAgarose I™ 250 g0710-250GAgarose I™ 500 g0710-500GAg

Page 4 - KLJKWKURXJKSXWVHTXHQFLQJ

99All You Need GenomicsElectrophoresisReagents & BuffersDescription Pk Cat. No.Agarose gel loading dye, 6X, containing 3 tracking dyes and 15% Fic

Page 5

ElectrophoresisReagents & Buffers100www.vwr.comDelivering High Quality & Superior PerformancePlease contact your local VWR sales office orgo to

Page 6

101All You Need GenomicsElectrophoresisMarkers & DyesDescription Pk Cat. No.100 bp ladder, sufficient for 50 lanes 250 µlK180-250UL100 bp Ready La

Page 7 - 0WENGKE#EKF2WTKƂECVKQP

ElectrophoresisMarkers & Dyes102www.vwr.comDescription Pk Cat. No.DNA MicroMarker™, sufficient for 50 lanes 100 µGE840-100UGDescription Pk Cat. No

Page 8

103All You Need GenomicsElectrophoresisMarkers & DyesDescription Pk Cat. No.EZ-Vision® 1 DNA dye kit, with dye migrating at ~10 bp in a 1% agarose

Page 9

ElectrophoresisMarkers & Dyes104www.vwr.comVWR.COMTALKING YOUR LANGUAGEThe local website with global reachDescription Pk Cat. No.GelRed™ 3X in wat

Page 10 - Nucleic Acid Purification

105All You Need GenomicsElectrophoresisGel TanksBuffer tank volume (ml) 250Capacity 1 large gel (12,5×13 cm), 2 small gels (12,5×6 cm) or 4 micro gels

Page 11 - FROM VWR INTERNATIONAL

ElectrophoresisGel Tanks106www.vwr.comBuffer volume (ml) 325Gel size W×L (mm) 60×75Sample capacity 32Unit W×L×H (mm) 130×240×65Recommended running vol

Page 12 - E.Z.N.A.® Blood DNA Kits

107All You Need GenomicsElectrophoresisGel TanksBuffer volume (ml) 450Gel size W×L (mm) 100×115Sample capacity 80Unit W×L×H (mm) 165×230×65Recommended

Page 13 - 11All You Need Genomics

9All You Need GenomicsNucleic Acid PurificationFastfilter Plasmid IsolationDescription Pk Cat. No.FastPlasmid Mini kit, 100 preps 100 Tests733-0175Fas

Page 14

ElectrophoresisGel Tanks108www.vwr.comBuffer volume (ml) 900Gel size W×L (mm) 128×150Sample capacity 112Unit W×L×H (mm) 200×320×70Recommended running

Page 15 - E.Z.N.A.® Tissue DNA kits

109All You Need GenomicsElectrophoresisGel TanksBuffer volume (ml) 1200Gel size W×L (mm) 150×150Sample capacity 120Unit W×L×H (mm) 215×335×70Recommend

Page 16

ElectrophoresisGel Tanks110www.vwr.comBuffer volume (ml) 2200Gel size W×L (mm) 200×200Sample capacity 168Unit W×L×H (mm) 270×475×80Recommended running

Page 17

111All You Need GenomicsElectrophoresisGel TanksBuffer volume (ml) 3000Gel size W×L (mm) 250×300Sample capacity 624Unit W×L×H (mm) 330×560×90Recommend

Page 18

ElectrophoresisGel Tanks112www.vwr.comModel Mini 10 Midi Plus 10 Midi Plus 15Buffer volume (ml) 225 300 500Gel size W×L (mm) 70×100 150×100 150×150Sam

Page 19

113All You Need GenomicsElectrophoresisPower SuppliesModel 300 V 250 VOutput voltage range/increments (V) 2 - 300/1 5 - 250/1Output current range/incr

Page 21

PCR Cloning 116Cloning

Page 22

CloningPCR Cloning116www.vwr.comPk Cat. No.40 AssaysPRME2201310Order your free copy online at http://eu.vwr.com/chemicalTHE CHEMICAL BRANDFROM VWR INT

Page 23 - E.Z.N.A.® MagBind® miRNA kits

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Page 24

Nucleic Acid PurificationGenomic DNA from Blood10www.vwr.comDescription Pk Cat. No.ArchivePure™ DNA Blood kit, for 30 ml blood 1KIT733-1014ArchivePure

Page 25 - E.Z.N.A.® Total RNA kits

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Page 26

Reagents 120Transfection

Page 27

TransfectionReagents120www.vwr.comDescription Pk Cat. No.jetPRIME® DNA and siRNA transfection reagent, 0,75 ml, with 60 ml jetPRIME® buffer 0,75 mlPPL

Page 28

121All You Need GenomicsTransfectionReagentsDescription Pk Cat. No.jetPEI®-FluoF DNA transfection reagent, 0,5 ml, with 50 ml NaCl solution 150 mM 500

Page 29 - VWR by Omega bio-tek

TransfectionReagents122www.vwr.comDescription Pk Cat. No.jetPEI®-FluoR DNA transfection reagent, 0,5 ml, with 50 ml NaCl solution 150 mM 500 µlPPLU106

Page 30 - Phase Lock Gel™

123All You Need GenomicsTransfectionReagentsINSTRUMENT SERVICEAND MAINTENANCEPlease contact your local VWR sales office for more informationHotlineRepa

Page 31

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Page 32

Reagents 126Electroporators, Cuvettes & Accessories 127Electroporation

Page 33

ElectroporationReagents126www.vwr.comE-BUSINESSMore than just a webshop for all of your laboratory needs Please visit us at: http://eu.vwr.com/e-busin

Page 34

127All You Need GenomicsElectroporationElectroporators, Cuvettes & AccessoriesInterfaces Digital user interfaceCharge time 5 seconds maximumVoltag

Page 35 - GE Healthcare

11All You Need GenomicsNucleic Acid PurificationGenomic DNA from BloodDescription Pk Cat. No.Ready PCR DNA column kit, 50 preps 50 Tests733-1050Descri

Page 36

ElectroporationElectroporators, Cuvettes & Accessories128www.vwr.comInterfaces Digital user interfaceCharge time 5 seconds maximumVoltagerangeLowV

Page 37 - FTA® Cards

129All You Need GenomicsElectroporationElectroporators, Cuvettes & AccessoriesDescription Pk Cat. No.HT 96 with ECM 830 generator, HT 200 plate ha

Page 38 - FTA® reagent and accessories

ElectroporationElectroporators, Cuvettes & Accessories130www.vwr.comAC parameters (alignment)Frequency (MHz) 1, fixedVoltage (VRMS) 0 - 75Duration

Page 39

131All You Need GenomicsElectroporationElectroporators, Cuvettes & AccessoriesDescription Pk Cat. No.In vivoGenetrode™, straight, 5 mm* 1 732-0010

Page 40

ElectroporationElectroporators, Cuvettes & Accessories132www.vwr.comDescription Pk Cat. No.Tweezertrode™ electrode*, 7 mm diameter 1732-0015Tweeze

Page 41 - Modifying Enzymes

133All You Need GenomicsElectroporationElectroporators, Cuvettes & AccessoriesParameter Optimisation chamber Production chamberVolume 2 ml 9 mlOut

Page 42 - Micrococcus

ElectroporationElectroporators, Cuvettes & Accessories134www.vwr.comDelivering High Quality & Superior PerformancePlease contact your local VW

Page 43 - Tritirachium album

Gel Documentation 136Spectrophotometers & Accessories 138Detection

Page 44

DetectionGel Documentation136www.vwr.comDescription Pk Cat. No.GenoMini gel documentation system 1730-3002Description Pk Cat. No.GenoSmart2 gel docume

Page 45

137All You Need GenomicsDetectionGel DocumentationVWR.COMTALKING YOUR LANGUAGEThelocalwebsitewithglobalreachDescription Pk Cat. No.GenoPlex CHEMI gel

Page 46 - DNA polymerase

Nucleic Acid PurificationGenomic DNA from Plant12www.vwr.comINSTRUMENT SERVICEAND MAINTENANCEPlease contact your local VWR sales office for more inform

Page 47 - 45All You Need Genomics

DetectionSpectrophotometers & Accessories138www.vwr.comLight source Long-life stabilised xenon flash lampDetector Twin CCD arrayWavelength range (

Page 48 - DNA polymerase MasterMixes

139All You Need GenomicsDetectionSpectrophotometers & AccessoriesModel Multiskan GO without cuvette port Multiskan GO with cuvette portLight sourc

Page 49 - MasterMix

www.vwr.comTable of contents140Standard High Purity Plasmid Isolation ... 6Endotoxin Free Plasmid Isolation ... 8(CUVƂNVGT

Page 51 - Hot Start PCR Reagents

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Page 52 - DNA polymerase, Fermentas

13All You Need GenomicsNucleic Acid PurificationGenomic DNA from Tissue and CellsDescription Pk Cat. No.illustra™ triplePrep kit, 50 preps 50 Tests28-

Page 53 - HotMasterMix

Nucleic Acid PurificationGenomic DNA from Tissue and Cells14www.vwr.comDescription Pk Cat. No.ArchivePure™ DNA Cell/Tissue kit, for 1g tissue or 2×10⁸

Page 54

15All You Need GenomicsNucleic Acid PurificationGenomic DNA from Yeast and BacteriaDescription Pk Cat. No.ArchivePure™ DNA Yeast and Gram-positive Bac

Page 55 - AccuPOL DNA polymerase

Nucleic Acid PurificationGenomic DNA from Soil, Stool and Water16www.vwr.comDescription Sample size Pk Cat. No.E.Z.N.A.® Soil DNA Mini kit 1 g 50 Test

Page 56

17All You Need GenomicsNucleic Acid PurificationGenomic DNA from FungiVWR.COMTALKING YOUR LANGUAGEThelocalwebsitewithglobalreachDescription Average yi

Page 57

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Page 58 - PCR Extender system

Nucleic Acid PurificationGenomic DNA from Insect and Mollusc18www.vwr.comE-BUSINESSMore than just a webshop for all of your laboratory needs Please vi

Page 59

19All You Need GenomicsNucleic Acid PurificationGenomic DNA PreparationDescription Pk Cat. No.illustra™ Genomiphi™ HY kit, 25 reactions 1KIT25-6600-22

Page 60

Nucleic Acid PurificationRNA from Blood20www.vwr.comDescription Sample size Pk Cat. No.PerfectPure™ RNA Blood kit 0,05 - 3 ml whole blood and buffy co

Page 61

21All You Need GenomicsNucleic Acid PurificationmiRNA/mRNA IsolationKit QuickPrep™ Micro mRNA purification kit QuickPrep™ mRNA purification kitNo. of

Page 62

Nucleic Acid PurificationmiRNA/mRNA Isolation22www.vwr.comDescription Pk Cat. No.illustra mRNA Purification Kit, 2 purifications (4 columns) 1KIT27-92

Page 63 - PCR Tubes

23All You Need GenomicsNucleic Acid PurificationRNA from Cells and Soft TissueDescription Sample size Pk Cat. No.PerfectPure™ RNA Cultured Cell kit 10

Page 64 - PCR Strips

Nucleic Acid PurificationRNA from Cells and Soft Tissue24www.vwr.comDescription Pk Cat. No.illustra™ RNAspin Mini isolation kit, 20 preps 20 Tests25-0

Page 65 - Caps for the PCR strips tubes

25All You Need GenomicsNucleic Acid PurificationRNA from Fatty TissueVWR.COMTALKING YOUR LANGUAGEThelocalwebsitewithglobalreachAverage yield Binding c

Page 66

Nucleic Acid PurificationRNA from Fribous Tissue26www.vwr.comDescription Sample size Pk Cat. No.PerfectPure™ RNA Fibrous Tissue kit 0,5 - 40 mg fibrou

Page 67

27All You Need GenomicsNucleic Acid PurificationTotal RNA ReagentsDescription Pk Cat. No.Isol-RNA Lysis Reagent™, 200 ml 200 ml733-1089Description Pk

Page 68

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Page 69

Nucleic Acid PurificationTotal RNA Reagents28www.vwr.comPLG HeavyAqueous phase High density sample (for example, high salt content)Organic phaseStanda

Page 70 - PCR seals

29All You Need GenomicsNucleic Acid PurificationRNA StabilisationDescription Pk Cat. No.RNase-free DNase set, 50 reactions 50 Tests733-1093E-BUSINESSM

Page 71

Nucleic Acid PurificationPCR Clean Up30www.vwr.comDescription Pk Cat. No.PCRExtract Mini kit, 50 preps 50 Tests733-2071PCRExtract Mini kit, 200 preps

Page 72 - Thermo Scientific

31All You Need GenomicsNucleic Acid PurificationPCR Clean UpDescription Pk Cat. No.illustra™ ExoStar™ 1-STEP kit 100 TestsUS77702illustra™ ExoStar™ 1-

Page 73

Nucleic Acid PurificationDNA/RNA Gel Extraction32www.vwr.comDescription Pk Cat. No.Agarose GelExtract Mini kit, 50 preps 50 Tests733-1988Agarose GelEx

Page 74 - PCR Workstations

33All You Need GenomicsNucleic Acid PurificationDNA/RNA Clean UpDescription Pk Cat. No.illustra™ ProbeQuant™ G-50 Micro Columns 5028-9034-08Descriptio

Page 75 - 73All You Need Genomics

Nucleic Acid PurificationDye Terminator Removal34www.vwr.comDescription Pk Cat. No.illustra AutoSeq™ G-50 columns 25027-5340-02Description Pk Cat. No.

Page 76 - PCR Cyclers

35All You Need GenomicsNucleic Acid PurificationDNA StorageDescription Pk Cat. No.FTA® CardsFTA® Classic Card 25512-1160FTA® Classic Card 100512-1079F

Page 77

Nucleic Acid PurificationDNA Storage36www.vwr.com512-1154Description Pk Cat. No.FTA® Elute Micro Card, 4 sample areas per card 25730-1364FTA® Elute Mi

Page 78

37All You Need GenomicsNucleic Acid PurificationDNA StorageDelivering High Qualit y & Superior Per formancePlease contact your local VWR sales offi

Page 79 - Real Time PCR

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Page 80 - Masterscript kit

© 2011 Thermo Fisher Scientific Inc. All rights reserved. You shouldn’t have to choose between purity and flexibility. Sensitivity and productivity. Thr

Page 81 - 79All You Need Genomics

DNA/RNA Modifying Enzymes 40Modifying Enzymes

Page 82

Modifying EnzymesDNA/RNA Modifying Enzymes40www.vwr.comDescription Pk Cat. No.DNA polymerase I, Klenow fragment fromE. coli200 EU A5217.0200DNA polyme

Page 83 - 81All You Need Genomics

41All You Need GenomicsModifying EnzymesDNA/RNA Modifying EnzymesVWR.COMTALKING YOUR LANGUAGEThelocalwebsitewithglobalreachDescription Pk Cat. No.Prot

Page 84

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Page 85

Standard PCR Reagents 44 Hot Start PCR Reagents 49 Direct PCR Reagents 52 High Fidelity PCR Reagents 53Long Range PCR Reagents 56dNTPs and Buffer

Page 86

PCRStandard PCR Reagents44www.vwr.comDescription Pk Cat. No.TaqDNA polymerase, 100 U (40 - 100 reactions of 50 µl) 100 733-0105TaqDNA polymerase, 250

Page 87 - 85All You Need Genomics

45All You Need GenomicsPCRStandard PCR reagentsDescription Pk Cat. No.TaqDNA polymerase (native, without BSA), 200 units (5 U/µl) 1KIT FERMEP0281TaqDN

Page 88 - PerfeCTa® qPCR ToughMix™

PCRStandard PCR reagents46www.vwr.comDescription Pk Cat. No.DreamTaq™ PCR master mix (2X), 4×1,25 ml (for 200 reactions of 50 µl) 5 mlFERMK1071DreamTa

Page 89 - 87All You Need Genomics

47All You Need GenomicsPCRStandard PCR reagentsDescription Pk Cat. No.illustra™ PuReTaq Ready-To-Go™ PCR Beads (0,2 ml tubes/multiwell plate) 96 Tests

Page 90

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Page 91 - 89All You Need Genomics

PCRStandard PCR reagents48www.vwr.comDescription Pk Cat. No.MasterTaq kit, 250 U (100-250 reactions of 50 µl) 250733-0114MasterTaq kit, 500 U (200-500

Page 92

49All You Need GenomicsPCRHot Start PCR ReagentsDescription Pk Cat. No.PerfectTaq™ Plus DNA polymerase, sufficient for 40×50 µl reactions 50 EU733-206

Page 93

PCRHot Start PCR reagents50www.vwr.comDescription Pk Cat. No.Maxima™ Hot StartTaqDNA polymerase, 100 units (5 U/µl) 1KIT FERMEP0601Maxima™ Hot StartTa

Page 94

51All You Need GenomicsPCRHot Start PCR reagentsDescription Pk Cat. No.illustra™ Hot Start Master Mix, 100 reactions 1KIT25-1500-01Description Pk Cat.

Page 95

PCRDirect PCR Reagents52www.vwr.comDescription Pk Cat. No.PCR directly from plantsPhire® Plant Direct PCR kit, 200 reactions (50 µl each) 1KITADVAF-13

Page 96

53All You Need GenomicsPCRHigh Fidelity PCR ReagentsDescription Pk Cat. No.Exact polymerase, sufficient for 40 reactions 100 EU733-0993Exact polymeras

Page 97

PCRHigh Fidelity PCR reagents54www.vwr.comDescription Pk Cat. No.PfuDNA polymerase (recombinant), 100 units (2,5 U/µl) 1KIT FERMEP0501PfuDNA polymeras

Page 98

55All You Need GenomicsPCRHigh Fidelity PCR reagentsDescription Pk Cat. No.Phusion® High-Fidelity PCR kit, 50×50 µl reactions 1KITFINRF-553SPhusion® H

Page 99

PCRLong Range PCR Reagents56www.vwr.comDescription Pk Cat. No.PCR Extender system, 100 U (50 - 100 reactions) 100733-0189PCR Extender system, 200 U (1

Page 100 - Reagents & Buffers

57All You Need GenomicsPCRdNTPs and BuffersDescription Pk Cat. No.dNTP Mix, 200 µl (10 mM) 200 µl733-0130dNTP Mix, 1000 µl (10 mM) 1ml733-0131Descript

Page 101

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Page 102 - VWR COLLECTION

PCRdNTPs and Buffers58www.vwr.comDescription Pk Cat. No.10XTaqbuffer with Mg2+,1,8ml 1,8 ml 733-0122Description Pk Cat. No.5X TaqMaster PCR-Enhancer 1

Page 103 - DNA ladder, 1 kb

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Page 104 - DNA MicroMarker™

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Page 105 - Ethidium bromide

All You Need GenomicsPCRTubes61VWR offer a complete range of PCR tubes.Here we have made a selection for you of the most popular tubes. The plastic ar

Page 106 - TALKING YOUR LANGUAGE

www.vwr.comPCRStrips62PCR stripsECNDescription Pack size Profile Type of strips Cap ColourTubes & caps per strip732-4800 Abgene Strip of 8 PCR tub

Page 107 - 105All You Need Genomics

All You Need GenomicsPCRCaps63Here we have made a selection for you of the most popular caps for the PCR strips tubes. The plastic are designed, manuf

Page 108

www.vwr.comPCRPlatesPCR plates compatibility tableChoosing the right PCR plate for your PCR cycler and assay is important.WůĂƚĞŵŽĚĞůƌĞĐŽŵŵĞŶĚĂƟŽŶƐĂ

Page 109 - 107All You Need Genomics

All You Need GenomicsPCRPlatesApplied Biosystems / Life Technologies Biorad/MJ Eppendorf Stratagene Biometra Roche Veriti™ 3847000/ 7300/7500/7700/790

Page 110

www.vwr.comPCRPlatesThermo ScientificApplied Biosystems / Life TechnologiesCat.No. Description Profile ColourNumber of wells SkirtedPiko 24Piko 96Arkt

Page 111 - 109All You Need Genomics

All You Need GenomicsPCRPlatesApplied Biosystems / Life Technologies Biorad/MJ Eppendorf Stratagene Biometra RocheVeriti™ 3847000/ 7300/7500/7700/7900

Page 112

Standard High Purity Plasmid Isolation 6Endotoxin Free Plasmid Isolation 8(CUVƂNVGT2NCUOKF+UQNCVKQP Genomic DNA from Blood 10Genomic DNA from

Page 113 - 111All You Need Genomics

www.vwr.comPCRSeals68PCR sealsHere we have made a selection for you of the most popular PCR seals. PCR seal platesECNDescription pack size732-0498 Abg

Page 114

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Page 115 - Power supplies, Power Source

PCRHeat Sealers70www.vwr.comOperating humidity range (%) 20 - 80% non condensingOperating temperature range (°C) 18...30Temperature set range (°C) 125

Page 116

71All You Need GenomicsPCRHeat SealersVWR.COMTALKING YOUR LANGUAGEThelocalwebsitewithglobalreachAir input pressure (psi/bar) 80 psi (5,5 bar) - 87 psi

Page 117 - PCR Cloning 116

PCRPCR Workstations72www.vwr.comModel UV3 Cabinet UV3 WorkstationFilterPre-filterCarbon filterHEPA filterZerOzone catalyst gridPre-filterCarbon filter

Page 118

73All You Need GenomicsPCRPCR CyclersModel ³Prime ³PrimeX ³PrimeGBlock accuracy (°C) <±0,25* <±0,25* <±0,25*Block homogeneity (°C) <±0,3*

Page 119 - >lXiXek\\[

PCRPCR Cyclers74www.vwr.com732-0663 732-1021Model Prime PrimeGBlock accuracy (°C) <±0,2* <±0,2*Block homogeneity (°C) <±0,3* <±0,3*Gradien

Page 120 - RGTHQTOCPEG

75All You Need GenomicsPCRPCR CyclersE-BUSINESSMore than just a webshop for all of your laboratory needs Please visit us at: http://eu.vwr.com/e-busin

Page 121 - Transfection

PCRPCR Cyclers76www.vwr.comOrder your free copy online at http://eu.vwr.com/chemicalTHE CHEMICAL BRANDFROM VWR INTERNATIONALModel TCP0024 TCP0096Block

Page 122

cDNA Synthesis 78SYBR Green qPCR 82TaqMan Probe qPCR 86One -Step TaqMan qRT-PCR 89One -Step SYBR qRT-PCR 89Reference Gene Assays 90HRM 91Hydrol

Page 123 - 121All You Need Genomics

Nucleic Acid PurificationStandard High Purity Plasmid Isolation6www.vwr.comDescription Pk Cat. No.PerfectPrep™ Spin Mini kit, 50 preps 50 Tests733-206

Page 124

Real Time PCRcDNA Synthesis78www.vwr.comDescription Pk Cat. No.Masterscript kit, 50 reactions 50 Tests733-0108Description Pk Cat. No.DyNAmo™ cDNA Synt

Page 125 - AND MAINTENANCE

79All You Need GenomicsReal Time PCRcDNA SynthesisDescription Pk Cat. No.M-MuLV Reverse Transcriptase, 5000 units (20 U/µl) 1KITFERMEP0351Activity, %T

Page 126 - (14.+37+&*#0&.+0)

Real Time PCRcDNA Synthesis80www.vwr.comDescription Pk Cat. No.Ready-To-Go™ T-Primed First-Strand kit 50 Tests27-9263-01Description Pk Cat. No.Ready-T

Page 127 - Electroporation

81All You Need GenomicsReal Time PCRcDNA SynthesisDescription Pk Cat. No.Phusion® RT-PCR kit, 20 reactions 1KITFINRF-546SPhusion® RT-PCR kit, 100 reac

Page 128 - E-BUSINESS

Real Time PCRSYBR Green qPCR82www.vwr.comINSTRUMENT SERVICEAND MAINTENANCEPlease contact your local VWR sales office for more informationHotlineRepairM

Page 129 - Electroporation cuvettes

83All You Need GenomicsReal Time PCRSYBR Green qPCRDelivering High Quality & Superior PerformancePlease contact your local VWR sales office orgo to

Page 130

Real Time PCRSYBR Green qPCR84www.vwr.comVWR.COMTALKING YOUR LANGUAGEThe local website with global reachDescription Pk Cat. No.DyNAmo™ Flash SYBR® Gre

Page 131 - 129All You Need Genomics

85All You Need GenomicsReal Time PCRSYBR Green qPCRdR/dT0.30.60.91.21.51.82.1858075706590Temperature (°C)Fluorescence (norm)00.20.40.60.81.01.21.44 8

Page 132

Real Time PCRTaqMan Probe qPCR86www.vwr.comDescriptionPkCat. No.PerfeCTa® qPCR FastMix® II without ROXPerfeCTa® qPCR FastMix II®, 250×20 µl reactions

Page 133 - Electrodes, Tweezertrodes™

87All You Need GenomicsReal Time PCRTaqMan Probe qPCRDescription Pk Cat. No.qPCR SuperMix, 50 reactions50Assays733-1272qPCR SuperMix, 200 reactions200

Page 134 - Electrodes, 2-Needle Array™

7All You Need GenomicsNucleic Acid PurificationStandard High Purity Plasmid IsolationDescription Pk Cat. No.illustra™ plasmidPrep Midi Flow kit, 25 pr

Page 135 - 133All You Need Genomics

Real Time PCRTaqMan Probe qPCR88www.vwr.comDescription Pk Cat. No.DyNAmo™ Flash Probe qPCR kit, 40×50 µl reactions 1 KITFINRF-455SDyNAmo™ Flash Probe

Page 136

89All You Need GenomicsReal Time PCROne -Step TaqMan/SYBR qRT-PCRDescription Pk Cat. No.One Step Fast qRT-PCR kits without passive reference dyeOne-St

Page 137 - Detection

Real Time PCRReference Gene Assays90www.vwr.comDescription Pk Cat. No.ACTB PerfeCTa® reference gene assay, Human ACTB (β-actin), 500×20 ul reactions 5

Page 138

91All You Need GenomicsReal Time PCRHRMDescription Pk Cat. No.AccuMelt™ HRM SuperMix (2×1,25 ml), sufficient for 250×20 µl reactions 2,5 mlQUNT95103-2

Page 139

Real Time PCRHydrolysis Probe Genotyping92www.vwr.comDescription Pk Cat. No.AccuStart™ Genotyping Toughmix® without ROXAccuStart™ qPCR ToughMix®, 250×

Page 140

93All You Need GenomicsReal Time PCRmicroRNA ProfilingDescription Pk Cat. No.qScript™ microRNA cDNA synthesis kit, 25×20 µl reactions 25 Assays733-201

Page 141 - 139All You Need Genomics

Real Time PCRqPCR Cyclers94www.vwr.comThermal blockBlock formats 24-well, 96-well (not interchangeable)Sample volume 10 - 50 µl (PikoReal® 24); 5 - 20

Page 142

95All You Need GenomicsReal Time PCRqPCR CyclersDescription Pk Cat. No.Piko® plate illuminator 1731-0358INSTRUMENT SERVICEAND MAINTENANCEPlease contac

Page 143

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Page 144 - '7466%OKP

Reagents & Buffers 98Markers & Dyes 101Gel Tanks 105Power Supplies 113Electrophoresis

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