VWR International 1325F Manuel d'utilisateur Page 87
- Page / 144
- Table des matières
- MARQUE LIVRES
Noté. / 5. Basé sur avis des utilisateurs
85All You Need Genomics
Real Time PCR
SYBR Green qPCR
dR/dT
0.3
0.6
0.9
1.2
1.5
1.8
2.1
8580757065
90
Temperature (°C)
Fluorescence (norm)
0
0.2
0.4
0.6
0.8
1.0
1.2
1.4
4 8 12 16 20 24 28 32 36 40
Cycle number
B
Derivative reporter (-Rn)
0
0.5
1.0
1.5
2.0
2.5
3.0
3.5
4.0
8580757065
90
Temperature (°C)
Δ
Rn
Cycle number
0
1.0
2.0
3.0
4.0
5.0
6.0
7.0
8.0
9.0
48
12 16 20
24
28 32 36 40
A
Description Pk Cat. No.
Maxima® SYBR Green qPCR master mix (2X), 2×1,25 ml (for 200 reactions of 25 µl), with ROX (50 µl) and 2×1,25 ml nuclease-free
water
2,5 ml
FERMK0251
Maxima® SYBR Green qPCR master mix (2X), 10×1,25 ml (for 1000 reactions of 25 µl), with ROX (250 µl) and 10×1,25 ml nuclease-
free water
12,5 ml
FERMK0252
Maxima® SYBR Green qPCR master mix (2X), 4×12,5 ml (for 4000 reactions of 25 µl), with ROX (1 ml) and 2×30 ml nuclease-free
water
1
FERMK0253
Maxima™ SYBR® Green qPCR master mix (2X), with ROX, Fermentas
Thermo Scientific
The Maxima™ SYBR Green qPCR master mix (2X) is a ready to use solution optimised for quantitative real time PCR and two-step real time
RT-PCR on most real time PCR machines. The master mix includes Maxima™ Hot Start
Taq
DNA polymerase, SYBR® Green dye I and
dNTPs in an optimised PCR buffer. ROX solution is provided in a separate vial for use with machines that require ROX as a passive reference
dye. Maxima™ Hot Start
Taq
DNA polymerase in combination with the optimised buffer ensures PCR specificity and sensitivity. The SYBR®
Green I intercalating dye allows for DNA detection and analysis without using sequence specific probes. dUTP is included in the mix for
optional carryover contamination control using uracil-DNA glycosylase (UDG). The use of Maxima™ SYBR Green qPCR master mix (2X) in
real time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates.
• Specificity – eliminates non specific amplification and formation of primer dimers
• Sensitivity – detects low copy number targets
• ROX solution provided in a separate tube
• Wide linear range – accurate quantification across 9 orders of magnitude
• Can be used on most real time PCR instruments
• Ready to use
Delivery information: Supplied with 50 µM ROX solution and nuclease-free water.
- #..;170''& 1
- +0641&7%6+10 2
- 6#$.'1(%106'065 2
- )'01/+%51.76+105 3
- KLJKWKURXJKSXWVHTXHQFLQJ 4
- 0WENGKE#EKF2WTKƂECVKQP 7
- Nucleic Acid Purification 10
- THE CHEMICAL BRAND 11
- FROM VWR INTERNATIONAL 11
- E.Z.N.A.® Blood DNA Kits 12
- 11All You Need Genomics 13
- 13All You Need Genomics 15
- E.Z.N.A.® Tissue DNA kits 15
- 21All You Need Genomics 23
- E.Z.N.A.® miRNA Isolation kit 23
- E.Z.N.A.® MagBind® miRNA kits 23
- 23All You Need Genomics 25
- E.Z.N.A.® Total RNA kits 25
- Total RNA Reagents 29
- VWR by Omega bio-tek 29
- Phase Lock Gel™ 30
- DNA/RNA Clean Up 35
- GE Healthcare 35
- 35All You Need Genomics 37
- FTA® Cards 37
- FTA® Elute Cards 38
- FTA® reagent and accessories 38
- Modifying Enzymes 41
- Micrococcus 42
- Tritirachium album 43
- DNA polymerase 46
- 45All You Need Genomics 47
- DNA polymerase MasterMixes 48
- 47All You Need Genomics 49
- PerfectTaq™ DNA polymerase 49
- MasterMix 49
- MasterTaq kits 50
- Hot Start PCR Reagents 51
- Maxima™ Hot Start 52
- DNA polymerase, Fermentas 52
- 51All You Need Genomics 53
- HotMasterMix 53
- 53All You Need Genomics 55
- Exact polymerase 55
- AccuPOL DNA polymerase 55
- PCR Extender system 58
- All You Need Genomics 63
- PCR Tubes 63
- PCR Strips 64
- Caps for the PCR strips tubes 65
- PCR seals 70
- Heat Sealers 72
- Thermo Scientific 72
- PCR Workstations 74
- 73All You Need Genomics 75
- PCR Cyclers 76
- Real Time PCR 79
- Masterscript kit 80
- 79All You Need Genomics 81
- 81All You Need Genomics 83
- 85All You Need Genomics 87
- PerfeCTa® qPCR FastMix® II 88
- PerfeCTa® qPCR ToughMix™ 88
- 87All You Need Genomics 89
- 89All You Need Genomics 91
- Reagents & Buffers 100
- VWR COLLECTION 102
- 101All You Need Genomics 103
- Electrophoresis 103
- DNA ladder, 100 bp 103
- DNA ladder, 1 kb 103
- DNA MicroMarker™ 104
- 103All You Need Genomics 105
- RNA ladder, 1 kb, RiboReady™ 105
- Ethidium bromide 105
- TALKING YOUR LANGUAGE 106
- 105All You Need Genomics 107
- 107All You Need Genomics 109
- 109All You Need Genomics 111
- 111All You Need Genomics 113
- 113All You Need Genomics 115
- Power supplies, Power Source 115
- PCR Cloning 116 117
- >lXiXek\\[ 119
- RTGEKUG 120
- RGTHQTOCPEG 120
- Transfection 121
- 121All You Need Genomics 123
- INSTRUMENT SERVICE 125
- AND MAINTENANCE 125
- (14.+37+&*#0&.+0) 126
- Electroporation 127
- E-BUSINESS 128
- 127All You Need Genomics 129
- Electroporator, ECM® 630 129
- Electroporation cuvettes 129
- 129All You Need Genomics 131
- 131All You Need Genomics 133
- Electrodes, Genetrodes™ 133
- Electrodes, Genepaddles 133
- Electrodes, Tweezertrodes™ 133
- Caliper electrodes 134
- Electrodes, 2-Needle Array™ 134
- 133All You Need Genomics 135
- Detection 137
- 139All You Need Genomics 141
- /QDKNHWPMOCZa/KP 144
- '7466%OKP 144
© 2020, manymanuals.fr. Tous droits réservés | 0.971 s |
Manymanuals.com
Manymanuals.de
Manymanuals.fr
Manymanuals.it
Manymanuals.pl
Manymanuals.cz
Manymanuals.es
Manymanuals-pt.com
Commentaires sur ces manuels