VWR International 1325F Manuel d'utilisateur Page 133
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131All You Need Genomics
Electroporation
Electroporators, Cuvettes & Accessories
Description Pk Cat. No.
In vivo
Genetrode™, straight, 5 mm* 1 732-0010
In vivo
Genetrode™, straight, 10 mm* 1 732-0011
I
novo
G
enetrode™, bent L-shaped, 5 mm* 1
7
32-0012
In ovo
Genetrode™, bent L-shaped, 3 mm* 1 732-0013
In ovo
Genetrode™, bent L-shaped, 1 mm* 1 732-0014
Description Pk Cat. No.
Genepaddles, 5×7 mm paddles* 1
732-0032
Genepaddles, 3×5 mm paddles* 1 732-0950
Accessories
Description Pk Cat. No.
Genetrode/Genepaddle holder 1
732-0028
Accessories
Description Pk Cat. No.
Genetrode/Genepaddle holder 1
732-0028
Electrodes, Genetrodes™
Electrodes, Genepaddles
Electrodes, Tweezertrodes™
BTX
BTX
BTX
Genetrodes™ are paired, reusable, needle style electrodes that are ideal for
in vivo
and
in ovo
electroporation applications, including drug and gene delivery.
Genepaddles are non invasive, paddle-style, reusable electrodes designed for
in vivo
and
in
vitro
applications, such as gene delivery (IVEGD) in mouse embryos. The electrodes are
placed anterior and posterior to the embryo following injection of the molecule of interest, and
an electroporation pulse is delivered.
Tweezertrodes™ are reusable, tweezer style electrodes for
in vivo
drug or gene delivery
applications. Tweezertrodes™ consist of a standard 11,5 cm long tweezer modified with
stainless steel circular disc electrodes inserted at the tip. Following injection of the molecule of
interest, the electrode discs grasp the target tissue and an electroporation pulse is delivered,
initiating pore formation and incorporation of the molecules into the tissue cells that are in
direct contact with the electrode.
• Five models to suit the size and shape of the target electroporation area
• Each model consists of a pair of electrodes configured as either straight or bent L-shaped
electrodes with gold tips
• The electrodes are placed into tissue following injection of the molecule of interest, and an
electroporation pulse is delivered using an electroporation generator
• The electric field introduced by the Genetrodes™ causes transient pores to form in the cells
of the tissue, allowing uptake of the molecules into cells
• Genetrodes™ are positioned in parallel at a predetermined gap in tissue using the
Genetrode/Genepaddle Holder
• Gold plated rectangular paddles, available in two sizes 3×5 mm or 5×7 mm
• Genepaddles may be positioned in parallel at a predetermined gap in tissue using the
Genetrode/Genepaddle Holder
• The gap between the electrodes may be adjusted up to 2 cm and have a positive electrode
indicator
• Choice of 7 mm or 10 mm diameter electrode
• Platinum Tweezertrodes™ are also available, please enquire for further details
Generator compatibility: ECM 830, ECM 2001
Generator compatibility: ECM 830, ECM 2001
Voltage range: 0 - 200 V DC
Voltage range: 0 - 200 V DC (do not use AC)
Pulse length range: 10 µsec to 99 msec in PBS
Pulse length range: 10 µsec to 99 msec in PBS
Pulse number range: 1 - 99 (depending on voltage and pulse length settings)
Pulse number range: 1 - 99 (depending on voltage and pulse length settings)
Genetrode™ holder electrode gap: 1 - 10 mm range
Genetrode™ holder electrode gap: 1 - 10 mm range
* Requires Genetrodes/Genepaddle holder, square post cables and coaxial banana plug cable
* Requires Genetrodes/Genepaddle holder, square post cables and coaxial banana plug cable
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- KLJKWKURXJKSXWVHTXHQFLQJ 4
- 0WENGKE#EKF2WTKƂECVKQP 7
- Nucleic Acid Purification 10
- THE CHEMICAL BRAND 11
- FROM VWR INTERNATIONAL 11
- E.Z.N.A.® Blood DNA Kits 12
- 11All You Need Genomics 13
- 13All You Need Genomics 15
- E.Z.N.A.® Tissue DNA kits 15
- 21All You Need Genomics 23
- E.Z.N.A.® miRNA Isolation kit 23
- E.Z.N.A.® MagBind® miRNA kits 23
- 23All You Need Genomics 25
- E.Z.N.A.® Total RNA kits 25
- Total RNA Reagents 29
- VWR by Omega bio-tek 29
- Phase Lock Gel™ 30
- DNA/RNA Clean Up 35
- GE Healthcare 35
- 35All You Need Genomics 37
- FTA® Cards 37
- FTA® Elute Cards 38
- FTA® reagent and accessories 38
- Modifying Enzymes 41
- Micrococcus 42
- Tritirachium album 43
- DNA polymerase 46
- 45All You Need Genomics 47
- DNA polymerase MasterMixes 48
- 47All You Need Genomics 49
- PerfectTaq™ DNA polymerase 49
- MasterMix 49
- MasterTaq kits 50
- Hot Start PCR Reagents 51
- Maxima™ Hot Start 52
- DNA polymerase, Fermentas 52
- 51All You Need Genomics 53
- HotMasterMix 53
- 53All You Need Genomics 55
- Exact polymerase 55
- AccuPOL DNA polymerase 55
- PCR Extender system 58
- All You Need Genomics 63
- PCR Tubes 63
- PCR Strips 64
- Caps for the PCR strips tubes 65
- PCR seals 70
- Heat Sealers 72
- Thermo Scientific 72
- PCR Workstations 74
- 73All You Need Genomics 75
- PCR Cyclers 76
- Real Time PCR 79
- Masterscript kit 80
- 79All You Need Genomics 81
- 81All You Need Genomics 83
- 85All You Need Genomics 87
- PerfeCTa® qPCR FastMix® II 88
- PerfeCTa® qPCR ToughMix™ 88
- 87All You Need Genomics 89
- 89All You Need Genomics 91
- Reagents & Buffers 100
- VWR COLLECTION 102
- 101All You Need Genomics 103
- Electrophoresis 103
- DNA ladder, 100 bp 103
- DNA ladder, 1 kb 103
- DNA MicroMarker™ 104
- 103All You Need Genomics 105
- RNA ladder, 1 kb, RiboReady™ 105
- Ethidium bromide 105
- TALKING YOUR LANGUAGE 106
- 105All You Need Genomics 107
- 107All You Need Genomics 109
- 109All You Need Genomics 111
- 111All You Need Genomics 113
- 113All You Need Genomics 115
- Power supplies, Power Source 115
- PCR Cloning 116 117
- >lXiXek\\[ 119
- RTGEKUG 120
- RGTHQTOCPEG 120
- Transfection 121
- 121All You Need Genomics 123
- INSTRUMENT SERVICE 125
- AND MAINTENANCE 125
- (14.+37+&*#0&.+0) 126
- Electroporation 127
- E-BUSINESS 128
- 127All You Need Genomics 129
- Electroporator, ECM® 630 129
- Electroporation cuvettes 129
- 129All You Need Genomics 131
- 131All You Need Genomics 133
- Electrodes, Genetrodes™ 133
- Electrodes, Genepaddles 133
- Electrodes, Tweezertrodes™ 133
- Caliper electrodes 134
- Electrodes, 2-Needle Array™ 134
- 133All You Need Genomics 135
- Detection 137
- 139All You Need Genomics 141
- /QDKNHWPMOCZa/KP 144
- '7466%OKP 144
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