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51All You Need Genomics
PCR
Hot Start PCR reagents
Description Pk Cat. No.
illustra™ Hot Start Master Mix, 100 reactions 1KIT
25-1500-01
Description Pk Cat. No.
illustra™ Hot Start Mix, Ready-To-Go™, 0,2 ml tubes, 96 reactions 96 Tests
28-9006-53
illustra™ Hot Start Mix, Ready-To-Go™, 0,5 ml tubes, 100 reactions 100 Tests
28-9006-46
illustra™ Hot Start Mix, Ready-To-Go™, 0,2 ml tubes, 5× 96 reactions 480 Tests
28-9006-54
Description Pk Cat. No.
HotMasterMix, 100 reactions 50 µl each 100 Tests
733-0120
HotMasterMix, 1000 reactions 50 µl each 1000 Tests
733-0121
illustra™ Hot Start Master Mix
illustra™ Hot Start Mix, Ready-To-Go™
HotMasterMix
GE Healthcare
GE Healthcare
5PRIME
Illustra™ Hot Start Master Mix combines high quality recombinant
Taq
DNA polymerase, a recombinant Hot Start activator protein, and
nucleotides in a proprietary reaction buffer. This ready to use mix provides robust and reliable performance for demanding PCR applications in
which high specificity and high sensitivity are essential to success. The mix does not contain
Taq
antibody and this eliminates the risk of
contamination from a mammalian source. Also, since the polymerase is not chemically inactivated, there is no extensive pre-cycling heating
step necessary; this reduces the chance of damaging precious DNA samples from heat-induced depurination. The net result from using
Illustra™ Hot Start Master Mix is a more robust and reliable amplification of the target amplicons with increased yield when compared to
conventional Hot Start Master Mix products.
Pre-formulated and pre-dispensed, freeze-dried PCR reagents mix including high quality PuReTaq™ DNA polymerase and a Hot Start
activator protein, for increased specificity and reproducibility of PCR amplifications. The Ready-To-Go™ beads are provided in either 0,5 mL
or 0,2 mL tubes that are compatible with most thermal cyclers.
HotMasterMix is a ready to use 2,5X reagent mix for performing high throughput PCR (based
on Hot Start/Cold Stop technology). The HotMasterMix guarantees the highest specificity and
consistency. The key component of the mix is the HotMaster
Taq
DNA polymerase that
consists of a pre-mixed HotMaster inhibitor that binds and blocks the substrate-binding site of
HotMaster
Taq
in a temperature-dependent manner. At temperatures above 60 °C, the
inhibitor is released and the HotMaster
Taq
becomes activated - Hot Start. At temperatures
below 60 °C, the inhibitor inactivates the
Taq
polymerase - Cold Stop, preventing non specific
primer annealing or primer-dimer formation. Unlike many conventional Hot Start PCR
techniques, such binding-releasing equilibrium is reversible throughout the PCR cycle.
• Recombinant Hot Start activator protein sequesters primers prior to PCR, effectively reducing non specific priming and primer-dimer
formation
• Quick Hot Start activation preserves sample integrity by avoiding extensive precycling heat treatment
• Avoids the risk of contamination from a mammalian source associated with Hot Start antibodies
• Convenient, ready to use mix saves time and reduces experimental variability
• Appropriate for any standard PCR reaction, such as multiplex PCR, and reactions exhibiting non specific amplification or primer-dimer
formation
• Recombinant Hot Start activator protein sequesters primers prior to PCR, effectively reducing non specific priming and primer-dimer
formation
• Avoids the risk of contamination from a mammalian source associated with Hot Start antibodies
• Quick Hot Start activation preserves sample integrity by avoiding extensive pre-cycling heat treatment
• Long-term ambient-temperature-stable: no freezer space required; less energy consumption for shipping and storage
• Validated for real time PCR
• Highly specific PCR
• Ready to use master mix format
• Convenient storage at 4 °C eliminates freeze and thaw cycles
• Minimal optimisation with self adjusting Mg₂+ buffer technology
Product comprises:
Taq
DNA polymerase (1,0 U/50 µl reaction), KCl, Mg₂+ and dNTPs.
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- Nucleic Acid Purification 10
- THE CHEMICAL BRAND 11
- FROM VWR INTERNATIONAL 11
- E.Z.N.A.® Blood DNA Kits 12
- 11All You Need Genomics 13
- 13All You Need Genomics 15
- E.Z.N.A.® Tissue DNA kits 15
- 21All You Need Genomics 23
- E.Z.N.A.® miRNA Isolation kit 23
- E.Z.N.A.® MagBind® miRNA kits 23
- 23All You Need Genomics 25
- E.Z.N.A.® Total RNA kits 25
- Total RNA Reagents 29
- VWR by Omega bio-tek 29
- Phase Lock Gel™ 30
- DNA/RNA Clean Up 35
- GE Healthcare 35
- 35All You Need Genomics 37
- FTA® Cards 37
- FTA® Elute Cards 38
- FTA® reagent and accessories 38
- Modifying Enzymes 41
- Micrococcus 42
- Tritirachium album 43
- DNA polymerase 46
- 45All You Need Genomics 47
- DNA polymerase MasterMixes 48
- 47All You Need Genomics 49
- PerfectTaq™ DNA polymerase 49
- MasterMix 49
- MasterTaq kits 50
- Hot Start PCR Reagents 51
- Maxima™ Hot Start 52
- DNA polymerase, Fermentas 52
- 51All You Need Genomics 53
- HotMasterMix 53
- 53All You Need Genomics 55
- Exact polymerase 55
- AccuPOL DNA polymerase 55
- PCR Extender system 58
- All You Need Genomics 63
- PCR Tubes 63
- PCR Strips 64
- Caps for the PCR strips tubes 65
- PCR seals 70
- Heat Sealers 72
- Thermo Scientific 72
- PCR Workstations 74
- 73All You Need Genomics 75
- PCR Cyclers 76
- Real Time PCR 79
- Masterscript kit 80
- 79All You Need Genomics 81
- 81All You Need Genomics 83
- 85All You Need Genomics 87
- PerfeCTa® qPCR FastMix® II 88
- PerfeCTa® qPCR ToughMix™ 88
- 87All You Need Genomics 89
- 89All You Need Genomics 91
- Reagents & Buffers 100
- VWR COLLECTION 102
- 101All You Need Genomics 103
- Electrophoresis 103
- DNA ladder, 100 bp 103
- DNA ladder, 1 kb 103
- DNA MicroMarker™ 104
- 103All You Need Genomics 105
- RNA ladder, 1 kb, RiboReady™ 105
- Ethidium bromide 105
- TALKING YOUR LANGUAGE 106
- 105All You Need Genomics 107
- 107All You Need Genomics 109
- 109All You Need Genomics 111
- 111All You Need Genomics 113
- 113All You Need Genomics 115
- Power supplies, Power Source 115
- PCR Cloning 116 117
- >lXiXek\\[ 119
- RTGEKUG 120
- RGTHQTOCPEG 120
- Transfection 121
- 121All You Need Genomics 123
- INSTRUMENT SERVICE 125
- AND MAINTENANCE 125
- (14.+37+&*#0&.+0) 126
- Electroporation 127
- E-BUSINESS 128
- 127All You Need Genomics 129
- Electroporator, ECM® 630 129
- Electroporation cuvettes 129
- 129All You Need Genomics 131
- 131All You Need Genomics 133
- Electrodes, Genetrodes™ 133
- Electrodes, Genepaddles 133
- Electrodes, Tweezertrodes™ 133
- Caliper electrodes 134
- Electrodes, 2-Needle Array™ 134
- 133All You Need Genomics 135
- Detection 137
- 139All You Need Genomics 141
- /QDKNHWPMOCZa/KP 144
- '7466%OKP 144
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