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PCR
Hot Start PCR reagents
50
www.vwr.com
Description Pk Cat. No.
Maxima™ Hot Start
Taq
DNA polymerase, 100 units (5 U/µl) 1KIT FERMEP0601
Maxima™ Hot Start
Taq
DNA polymerase, 500 units (5 U/µl) 1KIT FERMEP0602
Maxima™ Hot Start
Taq
DNA polymerase, 5×500 units (5 U/µl) 1KIT FERMEP0603
Description Pk Cat. No.
Phire® Hot Start II DNA polymerase, 200×50 µl reactions 1KIT
FINRF-122S
Phire® Hot Start II DNA polymerase, 1000×50 µl reactions 1KIT
FINRF-122L
Maxima™ Hot Start
Taq
DNA polymerase, Fermentas
Phire® Hot Start II DNA polymerase, Finnzymes
Thermo Scientific
Thermo Scientific
Maxima™ Hot Start DNA polymerase is designed to enhance the specificity, sensitivity and
yield of DNA amplification. In addition, the enzyme provides the convenience of reaction set-
up at room temperature. Maxima™ Hot Start DNA polymerase is a recombinant DNA
polymerase which has been chemically modified by the addition of heat-labile blocking groups
to its amino acid residues. The enzyme is inactive at room temperature, avoiding extension of
non specifically annealed primers or primer dimers and providing higher specificity of DNA
amplification. The functional activity of the enzyme is restored during a short 4-minute
incubation at 95 °C. The activated enzyme maintains the same functionality as DNA
polymerase: catalyses 5’→3’ synthesis of DNA, has no detectable 3’→5’ proofreading exonuclease activity, but possesses low 5’→3’
exonuclease activity. It exhibits deoxynucleotidyl transferase activity, which adds extra adenines at the 3’-end of PCR products. Before
activation, these two activities are not detectable. Applications include Hot Start PCR, high yield amplification of targets up to 3 kb from
genomic DNA and up to 5 kb from viral DNA, RT-PCR, highly specific amplification of complex genomic and cDNA templates, amplification of
low copy DNA targets, qPCR, multiplex PCR, and generation of PCR products for TA cloning.
Phire® Hot Start II DNA polymerase is faster, extremely robust, and capable of amplifying long DNA fragments with high yields. Phire® Hot
Start II DNA polymerase incorporates a dsDNA-binding domain which allows short extension times (10 to 15 s/kb), improves yields, and
increases fidelity 2-fold compared to
Taq
DNA polymerase. In addition, the Hot Start technology allows complete reactivation of the enzyme in
“zero-time” at standard cycling temperatures. This combination of features makes the polymerase an ideal solution for routine and high
throughput PCR applications. Phire® Hot Start II DNA polymerase delivers superior performance in conventional thermal cyclers as well as in
fast instruments, such as the Piko® Thermal Cycler.
• Quick Hot Start: No reactivation step
• Fast enzyme: Amplify four times faster than with Hot Start
Taq
• Robust: Minimal reaction optimisation due to high inhibitor tolerance
• High yields: Abundant products due to high efficiency
• Longer PCR products: Amplify significantly longer DNA fragments than with Hot Start
Taq
• High yield amplification of complex templates
• Four minute activation time
• High PCR specificity – reduced effects of mispriming and primer-dimer formation
• Enhanced PCR sensitivity
• Convenient room temperature PCR set-up
• Generates PCR products with 3’-dA overhangs
Delivery information: Supplied with 10X Hot Start PCR buffer and 25 mM MgCl₂.
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- Nucleic Acid Purification 10
- THE CHEMICAL BRAND 11
- FROM VWR INTERNATIONAL 11
- E.Z.N.A.® Blood DNA Kits 12
- 11All You Need Genomics 13
- 13All You Need Genomics 15
- E.Z.N.A.® Tissue DNA kits 15
- 21All You Need Genomics 23
- E.Z.N.A.® miRNA Isolation kit 23
- E.Z.N.A.® MagBind® miRNA kits 23
- 23All You Need Genomics 25
- E.Z.N.A.® Total RNA kits 25
- Total RNA Reagents 29
- VWR by Omega bio-tek 29
- Phase Lock Gel™ 30
- DNA/RNA Clean Up 35
- GE Healthcare 35
- 35All You Need Genomics 37
- FTA® Cards 37
- FTA® Elute Cards 38
- FTA® reagent and accessories 38
- Modifying Enzymes 41
- Micrococcus 42
- Tritirachium album 43
- DNA polymerase 46
- 45All You Need Genomics 47
- DNA polymerase MasterMixes 48
- 47All You Need Genomics 49
- PerfectTaq™ DNA polymerase 49
- MasterMix 49
- MasterTaq kits 50
- Hot Start PCR Reagents 51
- Maxima™ Hot Start 52
- DNA polymerase, Fermentas 52
- 51All You Need Genomics 53
- HotMasterMix 53
- 53All You Need Genomics 55
- Exact polymerase 55
- AccuPOL DNA polymerase 55
- PCR Extender system 58
- All You Need Genomics 63
- PCR Tubes 63
- PCR Strips 64
- Caps for the PCR strips tubes 65
- PCR seals 70
- Heat Sealers 72
- Thermo Scientific 72
- PCR Workstations 74
- 73All You Need Genomics 75
- PCR Cyclers 76
- Real Time PCR 79
- Masterscript kit 80
- 79All You Need Genomics 81
- 81All You Need Genomics 83
- 85All You Need Genomics 87
- PerfeCTa® qPCR FastMix® II 88
- PerfeCTa® qPCR ToughMix™ 88
- 87All You Need Genomics 89
- 89All You Need Genomics 91
- Reagents & Buffers 100
- VWR COLLECTION 102
- 101All You Need Genomics 103
- Electrophoresis 103
- DNA ladder, 100 bp 103
- DNA ladder, 1 kb 103
- DNA MicroMarker™ 104
- 103All You Need Genomics 105
- RNA ladder, 1 kb, RiboReady™ 105
- Ethidium bromide 105
- TALKING YOUR LANGUAGE 106
- 105All You Need Genomics 107
- 107All You Need Genomics 109
- 109All You Need Genomics 111
- 111All You Need Genomics 113
- 113All You Need Genomics 115
- Power supplies, Power Source 115
- PCR Cloning 116 117
- >lXiXek\\[ 119
- RTGEKUG 120
- RGTHQTOCPEG 120
- Transfection 121
- 121All You Need Genomics 123
- INSTRUMENT SERVICE 125
- AND MAINTENANCE 125
- (14.+37+&*#0&.+0) 126
- Electroporation 127
- E-BUSINESS 128
- 127All You Need Genomics 129
- Electroporator, ECM® 630 129
- Electroporation cuvettes 129
- 129All You Need Genomics 131
- 131All You Need Genomics 133
- Electrodes, Genetrodes™ 133
- Electrodes, Genepaddles 133
- Electrodes, Tweezertrodes™ 133
- Caliper electrodes 134
- Electrodes, 2-Needle Array™ 134
- 133All You Need Genomics 135
- Detection 137
- 139All You Need Genomics 141
- /QDKNHWPMOCZa/KP 144
- '7466%OKP 144
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